Journal article
Integrating single-cell genomics pipelines to discover mechanisms of stem cell differentiation
S Shen, Y Sun, M Matsumoto, WJ Shim, E Sinniah, SB Wilson, T Werner, Z Wu, ST Bradford, J Hudson, MH Little, J Powell, Q Nguyen, NJ Palpant
Trends in Molecular Medicine | CELL PRESS | Published : 2021
Abstract
Pluripotent stem cells underpin a growing sector that leverages their differentiation potential for research, industry, and clinical applications. This review evaluates the landscape of methods in single-cell transcriptomics that are enabling accelerated discovery in stem cell science. We focus on strategies for scaling stem cell differentiation through multiplexed single-cell analyses, for evaluating molecular regulation of cell differentiation using new analysis algorithms, and methods for integration and projection analysis to classify and benchmark stem cell derivatives against in vivo cell types. By discussing the available methods, comparing their strengths, and illustrating strategies..
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Grants
Awarded by Australian Research Council
Funding Acknowledgements
This work has been supported by grant funding from the Australian Research Council (SR1101002 and DP170101217 to N.J.P. and DP180101405 to J.P.) as well as by the National Health and Medical Research Council (APP1143163 and APP1175781 to N. J.P and J.P.)